Figure 6. Enhanced age associated lipid and lipofuscin deposition in the sin-3;him-5mutants (a) sin-3;him-5 hermaphrodite and male as compared to worms from him-5(e1490) and wild-type N2 worms at day one and day ten of attaining adulthood. (b) Enhanced age associated death pigment lipofuscin was observed in the sin-3;him-5 hermaphrodite and male as compared to worms from him-5(e1490) and wild-type N2 worms at day one and day ten of attaining adulthood. Visualization was done using NIKON fluorescence microscope. Magnification 400X, scale: 500 µm. 30 worms per strain were visualized and the experiment was repeated thrice.