Research Paper Volume 10, Issue 12 pp 4141—4151

Figure 4. Naringin suppresses osteosarcoma cell proliferation and migration by inhibiting Zeb1. (A) MTT proliferation assay of MG63 cells expressing a control vector or Zeb1. Cells were incubated with 20 μmol/L of naringin or NaCl and assayed at the indicated times. Results represent the mean ± SD of three experiments done in triplicate. **P < 0.05, compared with NaCl. ## P < 0.05, compared with Zeb1 overexpressed group. (B) MTT proliferation assay of MG63 cells transfected with si-Zeb1 (Zeb1 silencing) or si-NC (negative control). Cells were treated with 20 μmol/L of naringin or NaCl and assayed at the indicated times. Results represent the mean ± SD of three experiments done in triplicate. **P < 0.05, compared with NaCl. (C) Results of Transwell migration assays (without Matrigel) performed in MG63 cells expressing control vectors or Zeb1. Cells were treated with 20 μmol/l of naringin or NaCl. **P < 0.05, compared with NaCl; ## P < 0.05, compared with Zeb1 overexpressed group. (D) Results of Transwell migration assays (without Matrigel) performed in MG63 cells transfected with si-Zeb1 (Zeb1 silencing) or si-NC (negative control). Cells were treated with 20 μmol/l of naringin or NaCl. **P < 0.05, compared with NaCl. (E, F) Western blot and real-time PCR assay results for Zeb1, Cyclin D1, bcl-2, and MMP2 expression in MG63 cells expressing Zeb1 or empty vector. Cells were incubated with 20 μmol/l of naringin or NaCl (control). **P < 0.05, compared with NaCl; ## P < 0.05, compared with Zeb1 overexpressed group. (G, H) Western blot and real-time PCR assay results for Zeb1, Cyclin D1, bcl-2, and MMP2 expression in MG63 cells transfected with si-Zeb1 or si-NC. Cells were treated with 20 μmol/l of naringin or NaCl. **P < 0.05, compared with NaCl.