Research Paper Volume 11, Issue 2 pp 386—400

Figure 2. GPR109A is expressed in Hepatocytes. (A) An image of human liver section obtained from the Human protein atlas (www.proteinatlas.org) shows moderate immunoreactivity for GPR109A in hepatocytes. (B) Immunofluorescence staining of liver sections from WT and Gpr109a^mRFP mice using anti-RFPtag antibody confirm receptor expression in liver. (C) GPR109A mRNA expression in different cell populations isolated from mouse liver was evaluated by qPCR assay. (D) GPR109A mRNA expression in different hepatocyte cell lines of mouse and human origin was evaluated by qPCR assay. (E) GPR109A mRNA expression in liver of 4, 12, 18 and 24-month-old WT mice. Data are presented as mean ± S.E.M for (n=3-6). *p<0.05 vs. 4-month old mice.