Epigenetic clock analysis of human fibroblasts <i>in vitro</i>: effects of hypoxia, donor age, and expression of hTERT and SV40 largeT

Mieko Matsuyama; David J. WuWong; Steve Horvath; Shigemi Matsuyama

doi:doi:10.18632/aging.101955

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Research Paper Volume 11, Issue 10 pp 3012—3022

Epigenetic clock analysis of human fibroblasts in vitro: effects of hypoxia, donor age, and expression of hTERT and SV40 largeT

Figure 5. The progression of DNAm age after more than 10 PD of fibroblasts from donors of different ages from. (A and B) The progression of DNAmAge^391CpG (A) and DNAmAge^353CpG (B) after more than 10 PD of cell culture is shown. Each panel shows the following information: (A and B) Changes of DNAm age after more than 10 PD. (C and D) The Y axis indicates the values of DNAmAge^391CpG/PD (C) and DNAmAge^353CpG/PD (D). The X-axis indicates the DNAm age at the beginning of this experiment. The impact of PD on the speed of DNAm age progression becomes greater when the starting DNAmAge^391CpG is older, but becomes lesser when the starting DNAmAge^353CpG is older. The dots with the same color are the results from the same cell line.