Research Paper Volume 11, Issue 12 pp 4216—4237

Figure 4. TCQA stimulated hair bulb cells proliferation. (A) Cell proliferation of human epidermal melanocytes (HEM) was assessed after 48 and 72 h treatment with various concentrations of TCQA. (B) Cell proliferation of human hair follicle dermal papilla cells (HFDPCs) was assessed after 48 and 72 h treatment with various concentrations of TCQA. (C) ATP content determination after treatment with 5 and 10 µM of TCQA and 0.1 µM of minoxidil (Minox) used as positive control. (D) Gene expression of ALPL (Alkaline Phosphatase) after 6 and 12 h treatment with 0, 10 µM TCQA, and 0.1 Minox. The mRNA level was quantified using TaqMan real-time PCR after treatment. Results represent the mean ± SD of three independent experiments. *Statistically significant (P ≤0.05) difference between control and treated cells. **Statistically significant (P ≤0.01) difference between control and treated cells. ##Statistically significant (P ≤0.01) difference between Minox-treated cells and TCQA-treated cells.