Research Paper Volume 11, Issue 14 pp 4876—4889

Figure 7. SLCO4A1-AS1 regulated PARD3 expression by sponging miR-508-3p. (A) The diagram illustrated the predicted and mutant 3’-UTR of PARD3; (B) The targeted miRNA levels were measured by qRT-PCR in SW620 cells; (C) PARD3 expression in SW620 cells after transfection with specific miRNA mimics or inhibitor (miR-508-3p, miR-26a,miR-486-5p) detected by Western blot. (D) Enrichment of SLCO4A1-AS1 and miR-508-3p by using AGO2 antibody in SW620 cells by RIP assay. (E) miR-508-3p binding sites in SLCO4A1-AS1 3’-UTR region were predicted by TargetScan; (F, G) Luciferase activity of HEK293T and SW620 cells after co-transfection with PARD3-3’UTR-WT (or Mut) and miR-508-3p or SLCO4A1-AS-3’UTR-WT (or Mut)and miR-508-3p detected by dual-luciferase assays; (H, I) miR-508-3p expressions with up- or down-regulation of SLCO4A1-AS1 expression in CRC cells by qRT-PCR.