Research Paper Volume 11, Issue 14 pp 5008—5034

Figure 5. Rb1 increases GABA level in the PFC in MPTP-treated mice. (A-a, b, and c) Three different imaging orientations of the structural scans of the mouse brain. (A-d) The regions of interest (ROIs) in a brain slice positioned in the transverse plane to access the maximum of the PFC. GABA-CEST, B₀, and B₁ maps were also acquired from this slice (thickness = 3 mm). (B) The top two panels show GABA-CEST maps of the whole brain in (a) the MPTP group and (b) the MPTP+Rb1 group. The bottom two panels show GABA-CEST maps of the ROIs in the PFC of (c) the MPTP group and (d) the MPTP+Rb1 treatment group. (C and D) Superimposed maps of the Z-spectrum and magnetization transfer ratio asymmetry (MTR_asym) spectrum between the MPTP group and the MPTP+Rb1 treatment group. (E) Quantification of GABA level in the PFC of the MPTP group and the MPTP+Rb1 treatment group. n = 5 per group. Results are expressed as the mean ± SEM. ^##P < 0.01 vs. MPTP group. Statistical significance was determined by a Student's t-test.