Research Paper Volume 11, Issue 16 pp 6336—6357

Figure 2. Effects of aging and L-CS on intracellular Ca²⁺stores and NCX activity in cortical neurons.(A) Time course of CCCP-stimulated Ca²⁺release from mitochondria. Traces represent the average response to a 3 min exposure to 5 µM CCCP (Control^Veh: n=226 cells and Control^L-CSn=140; Aged^Vehn=91 cells and Aged^L-CSn=67 cells obtained from 7-19 independent experiments). (B) Dot plots depict Ca²⁺peak obtained in the four study groups. (C) Dot plots depict Ca²⁺changes expressed as AUC (a.u.). (D) Time course of CPA-stimulated Ca²⁺release from the ER. Traces represent the average response to a 2 min exposure to 10 µM CPA (Control^Veh: n=50 cells and Control^L-CSn=33; Aged^Vehn=54 cells and Aged^L-CSn=48 cells obtained from 3-4 independent experiments). (E) Dot plots depict Ca²⁺peak obtained in the four study groups. (F) Dot plots depict Ca²⁺changes expressed as AUC (a.u.). (G) Time course of NCX activity imaged by stimulating exchanger reverse operational mode (Control^Veh: n=163 cells and Control^L-CSn=122; Aged^Vehn=106 cells and Aged^L-CSn=98 cells obtained from 2 independent experiments). (H) Dot plots depict Ca²⁺peak obtained in the four study groups. (I) Dot plots depict Ca²⁺changes expressed as AUC (a.u.). Means were compared by two-way ANOVA followed by Tukey post-hoc test. * indicates p<0.05, *** p<0.001.