Research Paper Volume 11, Issue 18 pp 7386—7401

Figure 6. Knockdown of lncRNA DLEU2 suppresses tumor growth and epithelial to mesenchymal transition in vivo. (A) The tumor size was obtained from nude mice; (B) The tumor weight was measured in transferred si-DLEU2 or NC group; (C–D) The tumor volume curve of nude mice treated with si-DLEU2 or si-NC was analyzed; (E) The expression of lncRNA DLEU2, miR-30c-5p and SOX9 were measured by qRT-PCR; (F) The expression of SOX9 was measured by western blotting; (G) The expression of Ki-67 was detected in tumor tissues by immunohistochemistry; (H) Western blot was applied to detect E-cadherin, N-cadherin, and Vimentin protein expression in tumor tissues; (I) A549-si-DLEU2 and A549-NC were labeled with firefly luciferase and injected into the abdominal cavity of nude mice (n=3). (J) Histogram showing the bioluminescent signal intensity detected using a noninvasive In Vivo Imaging System. ^*p<0.05, ^**p<0.01, ^***p<0.001, compared with NC group.