Research Paper Volume 11, Issue 18 pp 7416—7441

Figure 1. Auditory Brainstem Response (ABR) threshold and Wave I analysis in KI mice. (A) Auditory Brainstem Responses (ABR) of p.V37I knock-in mice (Homozygous, red dotted line, Mean ± SEM) and age-matched wild-type mice (wt, black line, Mean ± SEM). Significant ABR threshold elevation was not observed until 25 weeks old, which started from 16 kHz (**P=0.005, F_(1.8)=14.629 at 25 weeks and *** P=0.000137, F_(1,8)=46.286 at 60 weeks, two-way ANOVA followed by Bonferroni post-test) and 22 kHz (**P=0.002, F_(1,8)=19.412844 at 25 weeks and **** P=0.000044, F_(1,8)=64.000 at 60 weeks, two-way ANOVA followed by Bonferroni post-test) gradually expanded to 11 kHz (*P=0.028, F_(1,8)=7.143 at 60 weeks, two-way ANOVA followed by Bonferroni post-test). A significant ABR threshold elevation in 4 kHz was observed at 25 weeks old (*P=0.010333, F_(1,8)=11.111, two-way ANOVA followed by Bonferroni post-test) but disappeared at 60 weeks old. ABR Wave I amplitude (B) and latency (C) of 16kHz and 22.6kHz in 60 week-old homozygous (red dotted line, Mean ± SEM) and wild-type (black line, Mean ± SEM) mice plotted as a function of sound levels. Amplitude did not differ between genotypes (both P>0.05, two-way ANOVA followed by Bonferroni post-test), while latency showed significant prolongation in homozygous mice (*P<0.01, **P<0.001, two-way ANOVA followed by Bonferroni post-test). Animals lacked visible Wave I were excluded for amplitude and latency analysis.