Research Paper Volume 11, Issue 18 pp 7416—7441

Figure 11. IHC synapse and OHC count in KI mice after middle ear injection of KCl solution. (A) Representative confocal images of IHC CTBP2 puncta before and after KCl middle ear injection. (Scale Bar, 5μm). (B) Histograms summarizing the average number of CTBP2 puncta at different turns (Mean + SEM). Middle ear injection of KCl solution did not affect CTBP2 expression (P>0.05, two-way ANOVA followed by Bonferroni post-test). (C) Representative confocal images of hair cells before and after KCl middle ear injection. (Scale Bar, 20μm). (D) Lack of significant hair cell loss observed in either genotype when compared to baseline data (d0) (P>0.05, two-way ANOVA followed by Bonferroni post-test). Baseline images and data were obtained from the same group of 20-week-old animals as in Figures 6 and 9.