Research Paper Volume 11, Issue 18 pp 7473—7491

Figure 2. hnRNPR promoted cell proliferation, migration and invasion of gastric cancer cells. (A) The efficiency of hnRNPR knockdown or overexpression was detected by western blot in the indicated cells after transfection with shhnRNPR or plasmids. GAPDH serve as the internal control. (B) CCK8 and (C) colony formation assays showed that hnRNPR knockdown suppressed cell growth in MGC-803 and AGS, while overexpression of hnRNPR promoted cell proliferation in HGC-27 and SGC-7901. (D) Migration assays and (E) invasion assays revealed that hnRNPR inhibition decreased cell migration and invasion abilities of MGC-803 and AGS, and hnRNPR overexpression increased cell migration and invasion abilities in HGC-27 and SGC-7901. Each experiment was performed in triplicate and repeated three times. P values were calculated with two-tailed unpaired Student’s t test. *, P<0.05, **, P<0.01.