Research Paper Volume 11, Issue 18 pp 7492—7509

Figure 8. Akt inactivation attenuates AEC senescence and pulmonary fibrosis in vivo. C57BL/6 male mice were randomly divided into vehicle group (n = 6), bleomycin group (n = 6) and inhibitor group (n=6). Mice in the vehicle group were intratracheally injected with 50 μl of 0.9% saline, and mice in the bleomycin group and inhibitor group were injected intratracheally with 50 μl of 5 mg/kg bleomycin. From the day of bleomycin injection, mice in the inhibitor group were treated daily with LY294002 (50 mg/kg) through intraperitoneal injection. On day 14 after bleomycin or saline treatment, lungs were harvested. (A, B) Expression of the P21^WAF1 senescent marker and the collagen1α fibrotic marker was detected by western blot analysis. Dots in the graph represent values for each individual mouse. *p < 0.05, ***p < 0.001. One-way ANOVA followed by Dunnett’s Multiple Comparison Test. (C) Representative results of HE and Masson staining of mouse lung tissues.