Research Paper Volume 11, Issue 20 pp 8777—8791

Figure 3. MALAT1 acts as a sponge of miR-34c to upregulate the expression of SATB2. (A) Prediction of binding sites between miR-34c and MALAT1 as well as those between miR-34c and SATB2 by RNA22. (B) The binding between miR-34c and SATB2 as assessed by luciferase activity assay. (C) The binding between miR-34c and MALAT1 as assessed by luciferase activity assay. (D) The binding of miR-34c and MALAT1 as assessed by RNA pull-down assay, * p < 0.05 vs. Bio-miR-34c-mut. (E) The binding of miR-34c and MALAT1 as assessed by RIP assay, * p < 0.05 vs. IgG. (F) The MALAT1 and miR-34c expression as well as the mRNA expression of SATB2 in osteoblasts (hFOB1.19) treated with miR-34c mimic detected by RT-qPCR. (G) The MALAT1 and miR-34c expression as well as the mRNA expression of SATB2 in osteoblasts (hFOB1.19) treated with oe-MALAT1 detected by RT-qPCR, * p < 0.05 vs. mimic-NC or oe-NC. (H) the protein expression of SATB2 in osteoblasts (hFOB1.19) treated with oe-MALAT1 or miR-34c mimic measured by Western blot analysis. Data were expressed with mean ± standard error. In Panel D and E, the one-way analysis of variance was used for data analysis, followed by Tukey’s post hoc test. in Panel B, C, F, G and H, the unpaired t test was used for data analysis. The experiment was repeated three times.