Research Paper Volume 11, Issue 18 pp 7639—7662

Figure 3. PTPN1 and MAP3K11 are direct targets of miR-125a-5p in HCC. (A) Three target prediction databases were used to predict the targets of miR-125a-5p; 131 common elements were identified. (B and C) These 131 target genes were analyzed with ClueGO and CluePedia in Cytoscape. (D and F) miR-125a-5p and its putative binding sequences in the 3′-UTRs of PTPN1 and MAP3K11. (E and G) miR-125a-5p overexpression inhibited, while knockdown increased, luciferase activity of the wild-type (WT), but not mutant (MT), PTPN1 or MAP3K11 3′-UTRs. Student’s t-test, mean ± SD, **P<0.01, ns: not statistically significant. (H) PTPN1 and MAP3K11 protein expression was assessed via Western blot in transfected Bel-7404 and SK-Hep1 cells.