Figure 1. Single-cell analyses of U87MG and U87MG-EGFRvIII cells. U87MG-EGFRvIII cells were less heterogeneous than U87MG cells. (A) Clustering analyses reveal ten subsets with cluster-specific genes and functions. The pie chart shows the percentage of each cluster. (B) The clustering results of U87MG-EGFRvIII cells (k=10) and the percentage of each cluster. (C) The clustering results with k values from two to ten. (D) The heatmap shows the gene expression of every single cell.