Figure 6. SPS does not influence neuronal and/or synaptic density. (A) Hippocampal sections were stained by Nissl staining to assess neuronal density (upper row scale bar=500μm, middle and lower row scale bar=100μm). (B–D) Quantitative analysis was performed, and SPS didn’t induce the neuronal death in CA1(B), CA3(C) and DG(D). (E–H) WB analysis showed no significant changes in the level of synaptophysin, synapsin1 and PSD95. Data are presented as mean ± SEM, n=3.