Research Paper Volume 11, Issue 20 pp 9075—9089

Figure 7. Activation of the Mst1-JNK pathway attenuates Sirt3-meidated mitochondrial protection. (A) ATP production was measured via ELISA. BV-2 cells were treated with LPS and/or transfected with Sirt3 adenovirus. Ani was used to activate the Mst1-JNK pathway. (B, C) ELISA was used to evaluate alterations in the mitochondrial respiratory complex in BV-2 cells after exposure to LPS stress. (D, E) ROS production was measured via immunofluorescence. BV-2 cells were treated with LPS and/or transfected with Sirt3 adenovirus. (F–H) Cellular antioxidant levels were determined via ELISA. I. Caspase-9 activity was detected via ELISA. *P<0.05 vs. control group; #P<0.05 vs. LPS+adenovirus-control group; @P<0.05 vs. LPS+adenovirus-Sirt3 group. N=3 independent experiments.