Research Paper Volume 11, Issue 22 pp 10266—10283

Figure 3. TMZ resistant cell lines U87TR and U251TR show higher chemotherapeutic resistance than that of the parent cell lines U87 and U251. (A) Morphological changes of drug-resistant cell lines U87TR and U251TR and parent cell lines U87 and U251 were observed by an optical microscope (× 200); (B) MTT assay was used to determine the cell proliferation capacity at the concentration of TMZ at 50 μg/mL; (C) Changes of parental cells and drug-resistant cells on IC50 of TMZ; (D) The expression of drug-resistant genes detected by western blot analysis; * P < 0.05 vs U87 cells or U251 cells; The data were all measurement data, represented by mean ± standard deviation. The comparison between the two groups was statistically analyzed by independent sample t test, and the experiment was repeated for three times.