Research Paper Volume 11, Issue 22 pp 10385—10408

Figure 4. RMF induced Ca²⁺ outflow from the endoplasmic reticulum to reduce intracellular MMP and ATP levels. (A) HUVECs were treated with RMF for 0 min, 2 min, 4 min and 4 min with a calcium channel inhibitor (BAPTA). After the addition of the Ca²⁺ probe Fluo-4-AM, the intracellular Ca²⁺ concentration was detected by flow cytometry. (B) The intracellular Ca²⁺ concentration in the same cells was detected following RMF treatment at 4-min intervals. (C) HUVECs were treated with RMF for 0 h, 2 h, 4 h, and 8 h. The intracellular ATP content was then detected. (D, E) After RMF treatment of HUVECs at 0 min, and 4 min in the presence and absence of the endoplasmic reticulum calcium channel inhibitor (BAPTA), changes in MMP levels were detected by flow cytometric analysis of the MMP dye, JC-1.