Research Paper Volume 11, Issue 22 pp 10385—10408

Figure 5. RMF increased the lifespan of C. elegans and reduced damage under stress conditions. (A, B) Changes of Ca2+ and ATP levels in C. elegans after RMF treatment. (C) The lifespan of 240 N2 C. elegans eggs following RMF treatment for 0 h/d (control), 1 h/d, 2 h/d and 4 h/d was recorded. (D) The lifespan of 240 N2 C. elegans eggs following RMF treatment for 0 h/d (control) and 2 h/d in the presence and absence of glucose was recorded. (E) Worms were treated with RMF for 0 h/d (control) and 2 h/d at 20°C for 4 days, and then exposed to thermal shock at 37°C. (F) Curves showing the non-paralyzed fraction nematodes in each group following treatment of C. elegans eggs with RMF for 0 h/d (control) and 2 h/d. CL4176 C. elegans were raised from the egg stage to the L3 stage at 15°C and then transferred to an environment at 25°C.