Research Paper Volume 11, Issue 22 pp 10532—10556

Figure 8. LH modulates the H2AX phosphorylation in somatic ovarian cells. (A) Representative IF for γH2AX in cultured ovarian somatic cells treated with 10 μM CS with/out 200 mIU/mL LH for the indicated times. Scale bar = 100 μm. (B) The graph reports the quantification of the percentage of cells positive for γH2AX. Data are shown as mean ± SEM of five experiments. Statistical differences vs control *p<0.05 ****p<0.0001; Cs+LH group vs CS group b = p<0.01 d = p<0.0001. (C, D) Representative WB of γH2AX in cells treated with CS with/out LH for the indicated times. Note a clear reduction of γH2AX level at 72 hrs. Data are expressed as mean ± SEM of three experiments. Statistical differences vs control **p<0.01 ***p<0.001 ****p<0.0001; statistical difference of CS group vs CS+LH group b = p<0.01 c = p<0.001.