Research Paper Volume 11, Issue 24 pp 11905—11921

Figure 4. KIF20A improves malignant behaviors in CRC. (A and B) Western blot and RT-qPCR analysis of KIF20A in constructed cell lines. Mock represents blank groups. Sh-NC represents lentivirus-mediated control groups. Sh-KIF20A represents lentivirus-mediated KIF20A silencing groups. (C and D) Western blot and RT-qPCR analysis of KIF20A in constructed cell lines. Mock represents blank groups. Lv-NC represents lentivirus-mediated control groups. Lv-KIF20A represents lentivirus-mediated KIF20A overexpression groups. (E) The relative growth rates in KIF20A knockdown CRCs were measured using CCK-8 analysis and compared between 3 groups at indicated times in two different cell lines. Data are presented as mean ± SEM. *P < 0.05; **P < 0.01. (F) The relative growth rates in KIF20A overexpression CRCs were measured using CCK-8 analysis and compared between three groups at indicated times in two different cell lines. Data are presented as mean ± SEM. *P < 0.05; **P < 0.01. (G–J) Colony formation of 4 different cell lines transfected with different treatments. Data are presented as mean ± SEM. *P < 0.05; **P < 0.01.