Figure 2. Plasma extracellular vesicle characterization. T cell and senescence markers are present on plasma EVs, but no differences were found between EVs from different age ranges (n=49 donors). (A) Representative cryoEM image of isolated EVs. (B) Representative figure of particle size distribution of EVs obtained by NTA. (C) Percentage of particles positive for EV characteristic markers assessed by flow cytometry. (D, E) Senescence markers on CD3+CD4+ and (F-G) on CD3+CD8+ EVs assessed by flow cytometry. Age range in years.