Figure 9. Effect of melatonin pretreatment on the expression and translocation of p65 in the different groups. Alexa Fluor 488 conjugated goat anti-p65 (A, D, G, J, M, P, S, V), DAPI (B, E, H, K, N, Q, T, W) and merged immunofluorescence (C, F, I, L, O, R, U, x) were observed under a fluorescence microscope.