Research Paper Volume 11, Issue 24 pp 12412—12427
CircRNA_100367 regulated the radiation sensitivity of esophageal squamous cell carcinomas through miR-217/Wnt3 pathway
Figure 3. Effects of circRNA_100367 on the proliferation and migration of KYSE-150R cells. (A) KYSE-150R cells were transfected with Sh-circRNA_100367 (or NC) and then irradiated with 0, 3, and 6 Gy X-ray. Cell ability was detected by MTT assay. (B) KYSE-150R cells were transfected with Sh-circRNA_100367 (or NC) and then irradiated with different dose X-ray. The proliferation of cells was evaluated by colony formation assay and survival fraction was analyzed by multi-target single-hit model. (C) KYSE-150R cells were transfected with Sh-circRNA_100367 (or NC). The migration of cells was assessed by wound healing assay. (D) KYSE-150R cells were transfected with Sh-circRNA_100367 (or NC). The protein levels of β-catenin, Vimentin, and Snail were detected by western blot. NC: the negative control of Sh-circRNA_100367. **p<0.01 vs. KYSE-150; #p<0.05, ##p<0.01 vs. KYSE-150+NC.