Research Paper Volume 11, Issue 24 pp 12546—12567

Figure 3. Sirt1 promotes p21 ubiquitination. (A) Isolated P1 CMs were transfected with Ad-NC or Ad-Sirt1. Whole cell lysates were immunoprecipitated with a p21 antibody and analyzed by Western blotting using an ubiquitin antibody (n=3). (B) Isolated P1 CMs were transfected with si-NC or si-Sirt1. Whole cell lysates were immunoprecipitated with a p21 antibody and analyzed by Western blotting using an ubiquitin antibody (n=3). (C) 8-week-old mouse hearts were injected with AAV9-NC or AAV9-Sirt1. Heart lysates were immunoprecipitated with a p21 antibody and analyzed by Western blotting using an ubiquitin antibody (n=5). Statistical significance was calculated using two-tailed unpaired Student’s t-test in (A–C). *p<0.05; data are presented as the mean ± S.E.M.