Research Paper Volume 12, Issue 7 pp 5948—5976

Figure 12. Silence of HOXA3 inhibited chondrocyte proliferation and promoted chondrocyte apoptosis. (A) The relative expression of HOXA3 after transfecting si-HOXA3 analyzed by RT-qPCR (n=3; ***P < 0.001). (B) The relative expression of HOXA3 after transfecting si-HOXA3 analyzed by western blot. (C) The effect of HOXA3 knockdown on cell proliferation detected by CCK8 assay (n=3; *P < 0.05, **P < 0.01). (D, F) The effect of HOXA3 knockdown on cell proliferation detected by EDU assay (n=3; *P < 0.05). (E, G) The effect of HOXA3 knockdown on cell apoptosis detected by flow cytometry assay (n=3; *P < 0.05). (H) Effects of HOXA3 knockdown on Col2a1, MMP13, BAX, and GAPDH protein levels detected by western blot.