Research Paper Volume 12, Issue 7 pp 5948—5976

Figure 3. miR-10a-5p acts as a significant contributing factor for OA. (A) The relative expression of COL2A1, MMP13, SOX9, and miR-10a-5p in control and IL-1β induced Hc-a analyzed by RT-qPCR (n=3; **P < 0.01, ****P < 0.0001). (B) The relative expression of miR-10a-5p after transfecting miR-10a-5p mimics, ***P < 0.001. (C) The effect of miR-10a-5p overexpression on cell proliferation detected by CCK8 assay (n=3; *P < 0.05, **P < 0.01, ***P < 0.001). (D, E) The effect of miR-10a-5p overexpression on cell proliferation detected by EDU assay (n=3; *P < 0.05). (F, G) The effect of miR-10a-5p overexpression on cell apoptosis detected by flow cytometry assay (n=3; ****P < 0.0001). (H) Effects of miR-10a-5p overexpression on Col2a1, MMP13, BAX, and GAPDH protein levels detected by western blot.