Research Paper Volume 12, Issue 10 pp 8858—8879

Figure 2. GSG2 knockdown inhibited bladder cancer cell proliferation, cell migration and induced cell apoptosis and cycle. (A) The knockdown efficiency of GSG2 in EJ and T24 cells was detected by qPCR. (B) The knockdown of GSG2 in EJ and T24 cells was verified by western blotting. (C) MTT assay was used to detect the effects of GSG2 knockdown on cell proliferation of EJ and T24 cells. (D) Flow cytometry was performed to evaluate the effects of GSG2 on cell apoptosis of EJ and T24 cells. (E) The effects of GSG2 knockdown on cell cycle distribution of EJ and T24 cells were examined by flow cytometry. Wound-healing (F) and Transwell (G) assays were utilized to reveal the effects of GSG2 knockdown on cell migration of EJ and T24 cells. The figures are representative data from at least three independent experiments. The data were expressed as mean ± SD (n ≥ 3), *P<0.05, **P<0.01, ***P<0.001.