Research Paper Volume 12, Issue 10 pp 8893—8912
Long noncoding RNA lnc-LOC645166 promotes adriamycin resistance via NF-κB/GATA3 axis in breast cancer
Figure 2. lnc-LOC645166 enhanced the chemoresistance of breast cancer cells by affecting cell proliferation and apoptosis. (A) RT–qPCR analysis of lnc-LOC645166 in 231/ADR and MCF-7/ADR cells transfected with sh-LOC645166-1, sh-LOC645166-2 or sh-NC for 48 h (n=3). (B) The effect of LOC645166 knockdown on the cell sensitivity to ADR of 231/ADR and MCF-7/ADR cells. (C, D) The effect of LOC645166 knockdown on the cell colony formation ability of 231/ADR and MCF-7/ADR cells under ADR treatment (231/ADR, 0.5μg/ml; MCF/ADR, 1μg/ml). (E, F) Flow cytometry analysis of Annexin V-stained 231/ADR and MCF-7/ADR cells transfected with sh-LOC645166-1, sh-LOC645166-2 or sh-NC upon adriamycin treatment (231/ADR, 0.5μg/ml; MCF/ADR, 1μg/ml) for 48 h (n=3). (G) Western blot analysis of the c-PARP,c-Caspase-3, p-STAT3 and t-STAT3 expression in 231/ADR and MCF-7/ADR cells transfected with sh-LOC645166-1, sh-LOC645166-2 or sh-NC upon adriamycin treatment (231/ADR, 0.5μg/ml; MCF/ADR, 1μg/ml) for 48 h (n=3). Data are shown as means ± SD. *p < 0.05, **p < 0.01, ***p < 0.001.