Research Paper Volume 12, Issue 8 pp 6733—6755

Figure 2. Metabolomics reveals a lower level of acylcarnitines in senescent vector PANC-1 cells, which is linked to reduced CPT1C expression. (A) PCA score plots of HILIC-ESI⁺-MS metabolomics profiles obtained from HILIC-ESI⁺-MS, n = 6/group. (B) Analysis of the relative response of acylcarnitine ions in senescent vector PANC-1 cells. Data are presented as the mean ± S.E.M, n = 6 (***p < 0.001). (C) Quantitative RT-PCR analysis of genes related to acylcarnitines. Data are presented as the mean ± S.E.M, n = 3 (ns indicates no significance, *p < 0.05, **p < 0.01, ***p < 0.001). The specific human primers to amplify corresponding mRNA were obtained from website of http://pga.mgh.harvard.edu/primerbank/ and PrimerDepot, and commercially available (Invitrogen) and shown in Supplementary Table 1. (D) Images and densitometric analysis of CPT1C protein bands of senescent vector PANC-1 cells. Data are presented as the mean ± S.E.M, n = 3 (**p < 0.01). See also Supplementary Figure 3.