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Research Paper Volume 12, Issue 8 pp 7056—7065

CO2 lattice laser reverses skin aging caused by UVB

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Figure 1. Effect of CO2 Lattice Laser on Fibroblasts. (A) Cell proliferation analyzed by MTT assay after treatment with 0, 1.25, 3.75 and 6.25 mJ/cm2 CO2 Lattice Laser. Data are shown as mean ± SEM.** P< 0.05 vs. 0 mJ/cm2 (B) ROS analyzed by ELISA in cells irradiated by CO2 lattice. Data are shown as mean ± SEM.** P< 0.05 vs. 0 mJ/cm2 (C) SOD expression analyzed by ELISA in cells irradiated by CO2 lattice laser. Data are shown as mean ± SEM.** P< 0.05 vs. 0 mJ/cm2 (D) MDA expression analyzed by ELISA in cells irradiated by CO2 lattice laser. Data are shown as mean ± SEM.** P< 0.05 vs. 0 mJ/cm2 (E, F) Western blotting and RT-PCR of SMAD3 in cells treated with 0, 1.25, 3.75 and 6.25 mJ/cm2 CO2 Lattice Laser; ** P< 0.05 vs. 0 mJ/cm2.