Research Paper Volume 12, Issue 8 pp 7056—7065

Figure 2. CO₂ lattice laser suppresses cell senescence induced by UVB. (A) Proliferation of fibroblasts treated with control, UVB, or UVB and 6.25 mJ/cm² CO₂ Lattice Laser. Data are shown as mean ± SEM;** P< 0.05 vs. control, ## P< 0.05 vs. UVB. (B) ELISA of ROS production in cells irradiated by control, UVB, or UVB and 6.25 mJ/cm² CO₂ Lattice Laser. Data are shown as mean ± SEM;** P< 0.05 vs. control, ## P< 0.05 vs. UVB. (C) ELISA of SOD in cells irradiated by control, UVB or UVB and 6.25 mJ/cm² CO₂ Lattice Laser. Data are shown as mean ± SEM;** P< 0.05 vs. control, ## P< 0.05 vs. UVB. (D) ELISA of MDA in cells irradiated by control, UVB or UVB and 6.25 mJ/cm² CO₂ Lattice Laser. Data are shown as mean ± SEM;** P< 0.05 vs. control, ## P< 0.05 vs. UVB (E) Cell cycle analyzed by flow cytometry after treatment with control, UVB or UVB and 6.25 mJ/cm² CO₂ Lattice Laser. Data are shown as mean ± SEM.** P< 0.05 vs. control, ## P< 0.05 vs. UVB. (F) Apoptosis was detected by Annexin V-PI after treatment with control, UVB or UVB and 6.25 mJ/cm² CO₂ Lattice Laser. Data are shown as mean ± SEM.** P< 0.05 vs. control, ## P< 0.05 vs. UVB. (G, H) Expression of SMAD3, CDK4, Bcl-2, and COL1 analyzed by western blotting and RT-PCR. Data are shown as mean ± SEM;** P< 0.05 vs. control, ## P< 0.05 vs. UVB.