Research Paper Volume 12, Issue 8 pp 7056—7065

Figure 3. CO2 Lattice Laser Reduces Cell Senescence through SMAD3. (A) Proliferation of fibroblasts treated with control, si-SMAD3 or si-SMAD3 and 6.25 mJ/cm² CO₂ Lattice Laser. Data are shown as mean ± SEM.** P< 0.05 vs. control, ## P< 0.05 vs. si-SMAD3. (B) ROS production in cells irradiated by control, si-SMAD3 or si-SMAD3 and 6.25 mJ/cm² CO₂ Lattice Laser. Data are shown as mean ± SEM.** P< 0.05 vs. control, ## P< 0.05 vs. si-SMAD3. (C) MDA expression in cells irradiated by control, si-SMAD3 or si-SMAD3 and 6.25 mJ/cm² CO₂ Lattice Laser. Data are shown as mean ± SEM.** P< 0.05 vs. control, ## P< 0.05 vs. si-SMAD3. (D) SOD expression in cells irradiated by control, si-SMAD3 or si-SMAD3 and 6.25 mJ/cm² CO₂ Lattice Laser. Data are shown as mean ± SEM.** P< 0.05 vs. control, ## P< 0.05 vs. si-SMAD3. (E, F) Expression of SMAD3, CDK4, Bcl-2, and COL1 analyzed by western blotting and RT-PCR in cells treated with control, si-SMAD3 or si-SMAD3 and 6.25 mJ/cm² CO₂ Lattice Laser. Data are shown as mean ± SEM;** P< 0.05 vs. control, ## P< 0.05 vs. si-SMAD3.