Research Paper Volume 12, Issue 8 pp 7056—7065

Figure 4. Therapeutic effect of CO₂ lattice laser on photoaging of rat skin. (A) HE staining of skin structures in control, UVB irradiation, and CO₂ lattice laser treatment groups. (B) Dermal thickness in control, UVB irradiation, and CO₂ lattice laser treatment groups. Data are shown as mean ± SEM;** P< 0.05 vs. control, ## P< 0.05 vs. UVB. (C) ROS production analyzed by ELISA in cells irradiated by control, UVB, or UVB and 50 mJ/cm² CO₂ Lattice Laser. Data are shown as mean ± SEM.** P< 0.05 vs. control, ## P< 0.05 vs. UVB. (D) ELISA of MDA in cells irradiated by control, UVB or UVB and 50 mJ/cm² CO₂ Lattice Laser. Data are shown as mean ± SEM.** P< 0.05 vs. control, ## P< 0.05 vs. UVB. (E) ELISA of SOD in cells irradiated by control, UVB or UVB and 50 mJ/cm² CO₂ Lattice Laser. Data are shown as mean ± SEM.** P< 0.05 vs. control, ## P< 0.05 vs. UVB. (F, G) Expression of SMAD3, CDK4, Bcl-2, and COL1 analyzed by western blotting and RT-PCR in control, si-SMAD3 or si-SMAD3 and 6.25 mJ/cm² CO₂ Lattice Laser groups. Data are shown as mean ± SEM.** P< 0.05 vs. control, ## P< 0.05 vs. UVB.