Research Paper Volume 12, Issue 9 pp 7786—7800

Figure 4. ACSL4 upregulation enhanced the stability of GLUT1 protein and reduced its ubiquitination. (A) After 12 hours of cell transfection with si-ACSL4 or OE-ACSL4, Huh-7 cells were treated with CHX (100 μg/ml) for 0, 1, 2, 4, 8 or 24 hours, and the western blotting assay was performed to detect GLUT1 expression. (B) An IP assay was used to detect the interaction between Ub and GLUT1 proteins after Huh-7 cells were transfected with si-ACSL4 or OE-ACSL4. (C) After 12 hours of cell transfection with si-ACSL4 or OE-ACSL4, SK-HEP-1 cells were treated with CHX (100 μg/ml) for 0, 1, 2, 4, 8 or 24 hours, and the western blotting assay was performed to detect GLUT1 expression. (D) IP assay was used to detect the interaction between Ub and GLUT1 protein in SK-HEP-1 cells. (^*P<0.05, si-ACSL4/OE-ACSL4 group compared with control group).