Research Paper Volume 12, Issue 10 pp 8939—8952
miR-378a-3p inhibits ischemia/reperfusion-induced apoptosis in H9C2 cardiomyocytes by targeting TRIM55 via the DUSP1-JNK1/2 signaling pathway
Figure 2. TRIM55 is a target of miR-378a-3p. (A) Complementary miR-378a-3p binding sequences in the 3′ UTR of SAMD1, TRIM55, FSTL1, MAPK1, DDAH1, and NPAS4. (B) SAMD1, TRIM55, FSTL1, MAPK1, DDAH1, and NPAS4 mRNA expression in H9C2 cardiomyocytes following I/R injury transfected with a miR-378a-3p mimic, inhibitor, or negative control (NC) was measured. (C) The dual-luciferase reporter assay was performed to confirm that TRIM55 is a target of miR-378a-3p. (D) H9C2 cardiomyocytes following 3 h ischemia and 24 h reperfusion were transfected with a miR-378a-3p mimic, inhibitor or NC and those without I/R injury were used as a control. Then, the protein expression of TRIM55 was measured. ***P < 0.001 compared with the control or NC. ###P < 0.001 compared with I/R + NC.