Research Paper Volume 12, Issue 13 pp 12726—12739

Figure 1. STK40 is highly expressed during HFSC differentiation into TA cells. (A) The growth of HFSCs observed under a microscope (5000 ×). (B) The expression of Alpha6 and CD34 determined by flow cytometry to sort HFSCs. (C) HFSC differentiation markers queried using immunofluorescence assay (400 ×). (D) Protein expression of STK40 normalized to GAPDH during HFSC differentiation determined using Western blot analysis. (E) Relative expression of STK40 during HFSC differentiation determined using RT-qPCR. * p < 0.05 vs. day 0; Measurement data were expressed as mean ± standard deviation. One-way ANOVA was utilized to compare data among multiple groups, followed by Tukey’s post hoc test. Cell experiments were conducted in triplicates.