Research Paper Volume 12, Issue 10 pp 9085—9102

Figure 8. Knockdown of lncRNA ZFAS1 blocks BLM-induced PF via regulation of the miR-150-5p/SLC38A1 axis. (A, B) HE and Masson staining were used to observe the morphological changes of lung tissues; (C, D) Immunohistochemistry assay was performed to detect the expression of E-cadherin and α-SMA in lung tissues; (D) Western blot was performed to measure the expression of E-cadherin, collagen I, FN1 and α-SMA in lung tissues; (E–G) The expression of miR-150-5p, SLC38A1, inflammatory cytokines in lung tissues were determined by RT-qPCR; (H) the level of ROS was measured by ROS kit; (I) The MDA content was measured by lipid peroxidation (MDA) assay kit; (J) RT-qPCR was performed to detect the level of GPX4 mRNA. ^**P<0.01, ^***P<0.001, compared with the control group; ^##P<0.01, compared with the BLM-treated group.