Research Paper Volume 12, Issue 12 pp 11386—11397

Figure 1. The isolation and identification of BMSCs and directional differentiation of BMSCs to steatoblast and osteoblasts. (A) The isolation and culture of BMSCs; (B) Clone formation ability of BMSCs stained by crystal violet staining; (C) Induction of steatoblast stained by Oil Red O staining; (D) Induction of osteoblasts stained by alizarin red staining; (E) Identification of surface markers of BMSCs by flow cytometry. White arrows indicate clone formation; Black arrows indicate lipid vesicles; Green arrows indicate calcified nodules.