Research Paper Volume 12, Issue 10 pp 9205—9223

Figure 2. Interaction network and analysis of hub genes. (A) The 20 most important hub genes were screened using the Cytoscape software plugin cytoHubba. (B) Hub genes and their co-expressed genes were analyzed using the cBioPortal. Nodes with a bold black outline represent hub genes. Nodes with thin black outlines represent co-expressed genes. (C) Biological processes functional annotation analysis of hub genes was performed using ClueGO and CluePedia. Different colors of nodes refer to the functional annotation of ontologies. Corrected P value <0.01 was considered statistically significant. (D) KEGG functional annotation analysis of hub genes was performed by ClueGO and CluePedia. Different colors of nodes refer to the functional annotation of ontologies. Corrected P value <0.01 was considered statistically significant. (E) Hierarchical clustering heatmap of the 20 most important hub genes was constructed from a TCGA cohort. Red indicates that the relative expression of genes was upregulated, green indicates downregulation, and black indicates that no significant change in gene expression was observed; gray indicates that signal strength was not high enough to be detected. Abbreviation: TCGA: the cancer genome atlas program; KEGG: Kyoto Encyclopedia of Genes and Genomes.