Research Paper Volume 12, Issue 10 pp 9405—9426

Figure 6. Sal improved MPTP- induced PC-12 cells. (A) Sal alone does not affect PC-12 cell viability but inhibits MPTP-induced the reduction of PC-12 cell viability. PC-12 cells (1 x 10⁴ cells/well) were exposed to a series concentrations of Sal (2, 10, 50 μM) for 24 h to determined the toxicity of Sal. PC-12 cells were incubated with Sal (2, 10, 50 μM) for 2 h, and then exposed to 500 μM MPTP for 24 h to determined the protective of Sal. The cell viability was measured by cell counting kit-8 (CCK-8) assay. (B) Sal inhibited MPTP-induced decreased TH and increased α-syn in PC-12 cells by Western blotting. The cells were incubated with Sal (2, 10, 50 μM) for 2 h, followed by stimulation with MPTP (500 μM) for 24 h. (C) Sal inhibited MPTP-induced increased of IL-1β, IL-18 and cleaved GSDMD in PC12 cells. Cells were incubated with Sal (2, 10, 50 μM) for 2 h, followed by stimulation with MPTP (500 μM) for 24 h. The levels of IL-1β and IL-18 in the supernatant were determined by ELISA, and protein of IL-1β, IL-18 and cleaved GSDMD in cells were determined by Western blotting. All data are represented as mean ± SD. ^# P < 0.05, ^## P < 0.01 vs. control group, *P < 0.05, **P < 0.01 vs MPTP group.