Research Paper Volume 12, Issue 10 pp 9534—9548
Dexmedetomidine inhibits LPS-induced proinflammatory responses via suppressing HIF1α-dependent glycolysis in macrophages
Figure 2. DEX inhibits glycolysis in LPS-treated macrophages. (A and B) BMDMs were seeded in Seahorse XFe96 cell culture microplates and treated with 100 ng/ml LPS and 1 μM DEX for 4 h. The real-time ECAR was recorded, and basal glycolysis and glycolytic capacity values were plotted. n = 5; mean ± SEM; * P < 0.05. (C) BMDMs were treated with 100 ng/ml LPS and 1 μM DEX for 4 h. Supernatants were collected, and the levels of glucose and lactate were measured. n = 3; mean ±SEM; * P < 0.05. (D) BMDMs were treated with 100 ng/ml LPS and 1 μM DEX for 4 h. The mRNA levels of GLUT1, HK2 and PFKFB3 were determined by RT-PCR. n = 3; mean ± SEM; * P < 0.05.