Research Paper Volume 12, Issue 10 pp 9549—9584
MiR-1908/EXO1 and MiR-203a/FOS, regulated by scd1, are associated with fracture risk and bone health in postmenopausal diabetic women
Figure 9. Prediction and verification of EXO1 and Fos expression regulated directly regulation by miR-1908 and miR-203a respectively. (A) Putative miR-203a and miR-1908 binding sites in the 3'-untranslated regions of FOS mRNA (predicted by miRanda) and EXO1 mRNA (predicted by miRWalk), respectively. (B) Luciferase reporter assays to evaluate FOS regulation by miR-203a and EXO1 regulation by miR-1908. (C) Transfection efficiency of miR-203a and miR-1908 in UM-Chor1 cells under the hyperglycemic circumstance. (D) UM-Chor1 cells co-transfected with pGL3-FOS-wt and miR-203a vs. those co-transfected with pGL3-FOS-mt and miR-NC; UM-Chor1 cells co-transfected with pGL3-EXO1-wt and miR-1908 vs. those co-transfected with pGL3-EXO1-mt and miR-NC.