Research Paper Volume 12, Issue 10 pp 9633—9657
MicroRNA-214-3p targets the PLAGL2-MYH9 axis to suppress tumor proliferation and metastasis in human colorectal cancer
Figure 2. MiR-214-3p suppresses CRC cell proliferation in vitro and in vivo. (A–B) CCK8 and colony-formation assays indicated that miR-214-3p inhibited the proliferation of CRC cells. (C) Western blot assays showed that miR-214-3p decreased the expression of cyclin D1, cyclin E and CDK4 and increased the expression of P27. (D–G) Tumors grew faster in the LV-miR-214-3p inhibitor group than in the LV-INC group. The tumor weights in the LV-miR-214-3p inhibitor group were higher than those in the LV-INC group. (H–I) IHC results indicated that the Ki-67 (a proliferation marker) index was increased remarkably when miR-214-3p was knocked down. The data are represented as the means±S.D. from at least three independent experiments. *p<0.05.