Research Paper Volume 12, Issue 10 pp 9633—9657
MicroRNA-214-3p targets the PLAGL2-MYH9 axis to suppress tumor proliferation and metastasis in human colorectal cancer
Figure 6. Silencing PLAGL2 significantly reverses the malignant process caused by the miR-214-3p inhibitor in CRC. (A–F) CCK8 and transwell assays indicated that PLAGL2 downregulation effectively reversed miR-214-3p inhibitor-induced proliferation (A–B) and the migration and invasion (C–F) abilities of CRC cells. (G) WB analysis revealed that the inhibitory effect of miR-214-3p on EMT was reversed by Sh-PLAGL2 transfection. (H–J) Silencing PLAGL2 reversed the effect of the miR-214-3p inhibitor on tumor growth in vivo. (K–L) Silencing PLAGL2 reversed the effect of the miR-214-3p inhibitor on tumor metastasis in vivo. The data are represented as the means±S.D. from at least three independent experiments. *p<0.05.