Research Paper Volume 12, Issue 10 pp 9633—9657

Figure 8. MiR-214-3p targets the PLAGL2-MYH9 axis to suppress tumor proliferation and metastasis in human colorectal cancer. (A–B) CCK8 assays indicated that PLAGL2 downregulation effectively reverses the miR-214-3p inhibitor-induced proliferation ability of CRC cells. (C) EDU assays indicated that PLAGL2 downregulation effectively reverses the miR-214-3p inhibitor-induced proliferation ability of CRC cells. (D–E) Transwell and wound-healing assays indicated that PLAGL2 downregulation effectively reversed miR-214-3p inhibitor-induced migration and invasion of CRC cells. (F–H) WB and qRT-PCR analyses revealed that the inhibitory effect of miR-214-3p on EMT was reversed by Si-MYH9 transfection. The data are represented as the means±S.D. from at least three independent experiments. *p<0.05.