Research Paper Volume 12, Issue 10 pp 9686—9713

Figure 11. OSE reformation post-ovulation in vivo is reduced with age. Representative image of Troma-1 IHC in (A) reproductively young ovaries and (B) reproductively old ovaries. Scale bars are 200 μm (A, B). Graphs showing the proportion of the ovarian surface that was (C) Troma-1 positive and (D) E-Cadherin positive. T-tests were performed for both parameters and asterisks denote significance (C: P = 0.01; D: P = 0.04). For Troma-1, N = 10 ovaries per age group, each ovary was analyzed in triplicate or quadruplicate (N = 34 measurements for reproductively young ovaries, N = 35 measurements for reproductively old ovaries). For E-Cadherin, N = 10 ovaries per age group (N = 20 measurements for reproductively young ovaries, N = 20 measurements for reproductively old ovaries).