Research Paper Volume 12, Issue 10 pp 9726—9744

Figure 3. Enhanced NF2 phosphorylation and subsequent nuclear translocation of YAP following PDGF-BB treatment or injury. (A) Nuclear and cytosolic-enriched fractions were prepared from VSMC, which was treated by PDGF-BB (30 ng/mL) for 48 h. The relative protein expression levels of p-NF2^Ser518, NF2 and YAP were determined by immunoblotting (n=5). (B) Nuclear and cytosolic-enriched fractions were prepared from arteries of C57BL/6J mice at day 28 after vascular injury. The relative protein expression levels of p-NF2^Ser518, NF2 and YAP were determined by immunoblotting (n=5). Data are shown as mean ± S.D. **P<0.01 and ***P<0.001 denote statistical comparison between the two marked groups, respectively.